產(chǎn)品名稱(chēng)：Dextrin Agarose Resin 6FF for MBP-Tagged Protein Purification， Dextrin填料（MBP蛋白純化填料）

貨號(hào)： P4160

品牌：Engibody

關(guān)鍵詞：Dextrin Resin, 麥芽糖結(jié)合蛋白（MBP）蛋白純化填料，用于MBP標(biāo)簽融合蛋白的純化。

Dextrin Beads 6FF is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).

Tested applications

Dextrin Beads 6FF provides convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Dextrin Beads 6FF based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.

Storage instruction

2°C to 8°C in 20% ethanol

Beads Diameter

90 µm

Binding Capacity

Approx. 7 mg MBP2*-paramyosin-δ-Sal/ml medium (Mr ~70 000,  multimer in solution)

Approx. 16 mg MBP2*-β-galactosidase/ml medium (Mr ~158 000,  multimer in solution)

Ligand

Dextrin

Matrix

Rigid, highly cross-linked 6% agarose beads

Maximum pressure

0.3 MPa, 3 bar

Chemical stability

Stable in all commonly used aqueous buffers, 0.5 M NaOH  (regeneration and cleaning)

Flow rate

Recommended ?ow velocity ≤ 150 cm/h, Maximum ?ow velocity < 300 cm/h

pH stability

working range > 7

short-term：2 to 13

Highlights

• Dextrin Beads 6FF is a robust, high resolution medium based on the 34 μm agarose matrix

• The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps

• Physiological conditions and mild elution preserve target Protein activity

• Dextrin Beads 6FF tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

Dextrin偶聯(lián)瓊脂糖珠6FF是麥芽糖結(jié)合蛋白（MBP）標(biāo)簽融合蛋白的親和純化介質(zhì)。

經(jīng)過(guò)測(cè)試的應(yīng)用

Dextrin偶聯(lián)瓊脂糖珠6FF提供了用麥芽糖結(jié)合蛋白（MBP）標(biāo)簽融合蛋白的方便親和純化。這種強(qiáng)健穩(wěn)定的基于葡聚糖珠6FF的培養(yǎng)基能夠在窄峰中洗脫目標(biāo)蛋白。

存儲(chǔ)說(shuō)明

20%乙醇中的2°C至8°C

Beads直徑

90µm

結(jié)合能力

約7 mg MBP2*-副肌球蛋白-δ-Sal/ml培養(yǎng)基（Mr~70 000，溶液中的多聚體）

約16 mg MBP2*-β-半乳糖苷酶/ml培養(yǎng)基（Mr~158000，溶液中的多聚體）

配體

Dextrin

基質(zhì)

剛性、高度交聯(lián)的6%瓊脂糖珠

最大壓力

0.3 MPa，3bar

化學(xué)穩(wěn)定性

在所有常用的水性緩沖液中穩(wěn)定，0.5M NaOH（再生和清洗）

流速

建議流速≤ 150 cm/h，最大流速<300 cm/h

pH穩(wěn)定性

工作范圍>7

短期：2至13

產(chǎn)品優(yōu)勢(shì)

•Dextrin偶聯(lián)瓊脂糖珠6FF是一種基于90μm瓊脂糖基質(zhì)的穩(wěn)健、高分辨率介質(zhì)

•大小均勻的小珠確保MBP標(biāo)記的蛋白質(zhì)以窄峰洗脫，從而最大限度地減少進(jìn)一步濃縮步驟的需要

•生理?xiàng)l件和溫和洗脫可保護(hù)靶蛋白活性

•Dextrin偶聯(lián)瓊脂糖珠6FF可耐受所有常用的水性緩沖液，并可使用0.5 M NaOH輕松再生，允許使用相同的柱進(jìn)行重復(fù)純化