P5601 His 蛋白純化試劑盒(磁珠法)
- 公司名稱 上海維景生物科技有限公司
- 品牌 其他品牌
- 型號(hào) P5601
- 產(chǎn)地 美國(guó)
- 廠商性質(zhì) 代理商
- 更新時(shí)間 2023/6/15 15:02:17
- 訪問次數(shù) 1252
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標(biāo)簽抗體偶聯(lián)beads，標(biāo)簽抗體，內(nèi)參抗體，WB實(shí)驗(yàn)服務(wù)，IP實(shí)驗(yàn)服務(wù)，ChIP實(shí)驗(yàn)服務(wù)
| 供貨周期 | 現(xiàn)貨 | 規(guī)格 | Kit |
|---|---|---|---|
| 貨號(hào) | P5601 | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
| 主要用途 | HA標(biāo)簽融合蛋白的富集純化 |
產(chǎn)品名稱：His tagged Protein Purification Kit (Ni-NTA Magnetic Beads, Soluble Protein)
品牌：Engibody
貨號(hào)：P5601
簡(jiǎn)介：His 蛋白純化試劑盒(磁珠法)，用于His標(biāo)簽融合蛋白的富集純化
Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). These tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates. Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Beads has been optimized to allow for high purity of the isolated His-tagged protein. Performance is equivalent to or better than Ni-NTA magnetic beads from other suppliers.
Description
His tagged Fusion Protein Purification Kit (Ni-NTA Magnetic Beads, Soluble Protein)
Tested applications
His-tagged Protein Purification ( by use Ni-NTA Magnetic Beads)
Components
Ni-NTA Magnetic Beads: 1ml
5g NiSO4;
6g imidazole;
10g EDTA
Binding Buffer: 100ml
Wash BufferL: 200ml
Storage instruction
2 - 8°C, do not freeze
Ni-NTA磁珠用于小規(guī)模親和純化以及重組His標(biāo)記蛋白的高通量篩選。聚his標(biāo)簽是的親和標(biāo)簽，通常由六個(gè)連續(xù)的（6xHis殘基）組成。這些標(biāo)簽融合的蛋白質(zhì)在許多不同的系統(tǒng)中過表達(dá)，最常見于細(xì)菌，并從細(xì)胞裂解物中純化。使用與histidine側(cè)鏈配位的帶鎳的NTA螯合物實(shí)現(xiàn)His標(biāo)記蛋白的純化。NTA螯合物包含四個(gè)金屬結(jié)合位點(diǎn)，允許低金屬離子浸出和高結(jié)合能力。Ni-NTA磁珠的方案已經(jīng)優(yōu)化，以允許分離的His標(biāo)記蛋白的高純度。性能優(yōu)于其他供應(yīng)商的Ni-NTA磁珠。
產(chǎn)品描述
his標(biāo)簽融合蛋白純化試劑盒（Ni-NTA磁珠，可溶性蛋白）
經(jīng)過測(cè)試的應(yīng)用
his標(biāo)簽融合蛋白純化（通過使用Ni-NTA磁珠）
Kit內(nèi)組份
Ni-NTA磁珠：1ml
5克硫酸鎳；
6克咪唑；
10克EDTA
Binding緩沖液：100ml
Wash緩沖液L:200ml
存儲(chǔ)說明
2-8°C，請(qǐng)勿冷凍
僅用于科研，不作用人體
采購中心
化工儀器網(wǎng)