GLP1R&GIPR Dual Reporter Cell GLP1R&GIPR Dual Reporter Cell
| I. Background | |
GLP-1R 與其他四種胰高血糖素受體（GCGR、GLP-2R、GIPR 和 GHRHR）一起屬于 促胰液素（B1 類）GPCR 家族，其內(nèi)源性配體是肽激素。 B1 類 GPCRs 在 N 端具有一個 較大的、結(jié)構(gòu)保守的胞外結(jié)構(gòu)域（ECD），由 120-160 個殘基組成，形成三層 α-β-β-α 折疊， 并由三個層間二硫鍵穩(wěn)定。 | |
| II. Description | |
GLP1R&GIPR Dual Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi) GLP1R&GIPR的信號轉(zhuǎn)導過程，原理見下圖所示。。
Figure 1. GLP1R&GIPR Dual Reporter Cell 細胞模型原理圖
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| III. Introduction | |
| Expressed gene: | GLP1R&GIPR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+ 2 μg/ml puro+ 5 μg/ml Blasticidin+ 200 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional assay for GLP1R&GIPR |
| Transducer: | Gs |
IV. Representative Data
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Figure 3. Dose response of Agonists in GLP1R&GIPR Dual Reporter Cell(C43).
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